CRISPR cas9 RNP
Genome-Editing Mutation Detection Kit
T7E1 enzyme cleaves heteroduplex DNA only.
Using the genomic DNA extracted from cells or tissues subjected to gene-editing processes as template, target DNA region that possibly contains mutations is amplified by High-Fidelity DNA polymerase. The PCR products are then denatured and annealed, followed by digestion with T7E1 enzyme which cleaves heteroduplex DNA only.
Genome-Editing Mutation Detection Kit provides a simple, reliable, and rapid method for detecting the mutations on genomic DNA extracted from cells or tissues subjected to CRISPR/Cas9 or other gene-editing processes. It employs the High-Fidelity DNA polymerase for amplifying the target DNA fragments, and the T7 Endonuclease I (T7E1) for recognizing and cleaving non-perfectly matched DNA (heteroduplex DNA).
Genome-Editing Mutation Detection Kit contains all reagents for DNA extraction, PCR amplification with high fidelity, and T7E1 enzyme digestion. It also contains positive control template DNA and its corresponding primers for troubleshooting. The DNA extracts and PCR products can be added directly into the subsequent reactions without extra purification, enabling the kit convenient to use.
Working mechanism of Genome-Editing Mutation Detection Kit: Using the genomic DNA extracted from cells or tissues subjected to gene-editing processes as template, target DNA region that possibly contains mutations is amplified by High-Fidelity DNA polymerase. The PCR products are then denatured and annealed, followed by digestion with T7E1 enzyme which cleaves heteroduplex DNA only. If mutations exist in target DNA, heteroduplex will be formed between unmutated DNA and mutated DNA, or between mutated DNA with different mutations. The mutated DNA is then cleaved by T7E1 into smaller fragments. Based on the concentrations of cleaved and uncleaved fragments, the efficiency of genome editing can be estimated.
Control Template provided in this kit contains both unmutated and mutated gene fragments. Amplicons of unmutated fragments are 525bp in length, and those of mutated fragments with 7nt deletion mutation are 518bp in length. The mutated DNA strand in the Heteroduplex DNA formed between the unmutated and the mutated DNA fragments is cleaved by T7E1 into two DNA fragments, 288bp and 230bp in length, respectively. Meanwhile, the homoduplex of either unmutated or mutated DNA could not be cleaved by T7E1. Therefore, after T7E1 digestion, positive control samples should contain DNA fragments of four different sizes (525bp, 518bp, 230bp, and 288bp). Usually, the 525bp and 518bp bands are nearly together when separated on agarose gels.
Katalog Nummer: 25-6050 DNA
Gene CRISPR Easy KO Kit
This kind of kits are used for human eukaryotic cell gene knockout experiments in vitro.
It employs multiple chemically synthesized and modified single guide RNAs (sgRNAs) to target the gene of interest, and utilizes Cas9-gRNA ribonucleoproteins (RNPs) to knockout the gene.
After the RNPs are transfected into cells, they will cleave the early exons of the target gene, leading to loss-of-function by fragment deletion in early exons.
If you do need another sort of gene knock-out kit , please feel free to ask us about.
USP47 CRISPR Easy KO Kit
Gene Symbol : USP47 // Species: human
NCBI Gene ID : 55031
Ensembl ID: ENSG00000170242 // LINK : https://www.ensembl.org/index.html?redirect=no
Uniprot ID: Q96K76
Product Contents
Chemically synthesized sgRNA (300pmol), LM RNP-specific transfection reagent, LM cell lysate, PCR primers, and sequencing primers.
10 reactions /1Target gene，
1Target gene /1.5mL tube
Key Advantages of USP47 CRISPR Easy KO Kit
High Efficiency Knockout – Utilizes chemically synthesized and modified sgRNAs with Cas9 RNPs for rapid and precise USP47 gene disruption.
Targeted Early Exon Cleavage – Ensures loss-of-function by fragment deletion in early exons.
Ready-to-Use Kit – Includes all necessary components: sgRNA, RNP transfection reagent, cell lysate, PCR and sequencing primers.
Minimal Off-Target Effects – Optimized sgRNA design reduces unintended gene editing.
Flexible Storage – Long-term storage at -80℃ (1 year) or short-term at -20℃ (6 months) with minimal activity loss.
Applications of USP47 CRISPR Easy KO Kit
Human eukaryotic cell gene knockout experiments in vitro
Functional genomics research
Disease mechanism studies
Drug target validation
Cell line engineering for research
Quality & Compliance
Specification Verification: All sample parameters are cross-checked, including biological characteristics, container type, and documentation.
Quality Assurance: Manufactured under ISO 13485 standards with complete batch traceability for reproducible research.
Reliable & Traceable: Validated for consistent performance across experiments, ensuring safe and dependable use.
Bulk & Wholesale Solution
We offer bulk supply and wholesale USP47 CRISPR Easy KO Kit options for research institutes, laboratories, and distributors.
Benefits include:
Competitive pricing for large-volume orders
Consistent batch quality for multiple experiments
Fast shipping with temperature-controlled logistics
Dedicated support for training and technical queries
Get in touch with us
Get in touch with us now to talk about your inquiries.
Please talk with Mark
+86 198 1376 3823
mark@tsktbiomed.com
Please talk with Tony
+86 151 0713 7823
tony@tsktbiomed.com
Please talk with Klaus
Customer service
+86 181 6278 5319
info@tsktbiomed.com
https://www.tsktbiotech.com/reception/pagedis/inquiry/471033.html
FOR RESEARCH USE ONLY
"ready-to-use" : USP30 ** / USP47 / TPP2 / HLA-DQB1 uvm. KO kits available.
please inquire : info@sansunbiologics.com
