Fluorescent probes
Neuronal fluorescent probes
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Artikelnummer |
Produktbeschreibung |
Preis pro Verpackungseinheit in Euro (€) zzgl. MwSt und Versandkosten *** |
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T-N3130 |
NerveRed C2 neuronal fluorescent probe. Store at -20℃, avoid light. |
329,60 € / 1 mg |
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T-N3140 |
NM4-64 neuronal fluorescent probe. Store at -20℃, avoid light. |
547,10 € / 1 mg |
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T-N3150 |
NerveGreen C4 neuronal fluorescent probe |
616,00 € / 5mg |
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***) Gerne erstellen wir Ihnen ein detailliertes Angebot für Ihre Einkaufsabteilung. |
Fluorescence spectrum:
Abs/Em (in membranes) : 510/750 nm
Abs/Em (in MeOH): 543/- nm, (emission in MeOH is too weak to measure)
Product introduction:
These probes are a series of cationic styrene based fluorescent dyes used to track the synaptic activity of neuromuscular connections or synapses. These dyes typically have lipophilic tails (two carbon chains) and highly hydrophilic heads with cations. The NerveRedTM series probes are dyes with three double bonds. Cationic styrene based dyes function by staining synaptic vesicles in an activity dependent manner. When dyes co incubate with cells or tissues, the aqueous part of the dye does not show fluorescence, while the lipophilic tail of the dye inserts into the cell membrane and exhibits strong fluorescence. After nerve stimulation, during endocytosis, the dye is encapsulated within the vesicles. Therefore, after washing away the dye attached to the cell surface, the strength of the fluorescence signal indicates the number of newly formed vesicles. On the contrary, during exocytosis, dyes and neurotransmitters are released together from the vesicles, leading to a decrease in fluorescence signals. Therefore, changes in fluorescence intensity reflect the situation of endocytosis/exocytosis or synaptic activity. The rate of fluorescence increase during endocytosis - the "binding rate" and the rate of fluorescence decrease during exocytosis - the "dissociation rate" vary depending on the type of dye. Typically, dyes with longer lipophilic tails and more double bonds have a higher affinity for membranes, resulting in higher binding rates and lower dissociation rates.
Method of use: This product is equivalent to FM4-64. NerveRed C2 dye cannot be used for staining fixed cells. NM series dyes can be selected for staining fixed cells.
Enzyme substrates based on FRET
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Fluorophore |
Quencher |
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FAM |
Dabcyl |
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Trp |
Dnp |
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Abz |
Dnp |
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Mca |
Dnp |
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Dabcyl |
Glu (EDANS) |
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EDANS |
Dabcyl |
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***) Gerne erstellen wir Ihnen ein detailliertes Angebot für Ihr Protease / Enzyme Substrate - FRET! |
EdU Cell Proliferation Assay Kit / EdU Flow Cytometry Assay Kit
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Artikelnummer |
Produktbeschreibung |
Preis pro Verpackungseinheit in Euro (€) zzgl. MwSt und Versandkosten *** |
|
SF647A |
SF647A EdU Cell Proliferation Assay Kit (Far-red Fluorescence. Store at -20℃, avoid light. |
229,60 € / 100 T |
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T-CA1174 |
SF594 EdU Cell Proliferation Assay Kit (Red Fluorescence) |
229,60 € / 100 T |
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T-CA1173-B |
SF647A EdU Flow Cytometry Assay Kit (Far Red Fluorescence) |
358,00 € / 20 T |
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APC / PE / Antibody Labeling Kits
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Artikelnummer |
Produktbeschreibung |
Preis pro Verpackungseinheit in Euro (€) zzgl. MwSt und Versandkosten *** |
|
T-EX7036 |
APC Antibody Labeling Kits; Store at -20℃, avoid light |
391,20 € / 100 µg |
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T-EX7035 |
PE Antibody Labeling Kits; Store at -20℃, avoid light |
391,20 € / 100 µg |
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T-EX7036: Allophycocyanin (APC) is a blue fluorescent protein, which has an excitation wavelength of 640 nm and an emission wavelength of 661 nm. The interchain disulfide bonds of the (IgG, IgY) antibody can be cleaved to generate active cysteine residues, which are then conjugated with the preactivated APC to obtain the APC-antibody conjugate. |
T-EX7035: PE / R-Phycoerythrin Conjugation Kit | |
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Tandem Dye Antibody Labeling
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Artikelnummer |
Produktbeschreibung |
Preis pro Verpackungseinheit in Euro (€) zzgl. MwSt und Versandkosten *** |
|
T-EX7023 |
APC - SF750 Tandem Dye Antibody Labeling Kits; Store at -20℃, avoid light |
960,60 € / 500 µg |
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T-EX7025 |
APC - Cy5.5 Tandem Dye Antibody Labeling Kits; Store at -20℃，avoid light |
499,10 / 100 µg |
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T-EX7032 |
PE - Cy7 Tandem Dye Antibody Labeling Kits; Store at -20, avoid light |
499,10 / 100 µg |
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T-EX7023/25: Allophycocyanin (APC) is a blue fluorescent protein, which has an excitation wavelength of 640 nm and an emission wavelength of 661 nm. The interchain disulfide bonds of the (IgG, IgY) antibody can be cleaved to generate active cysteine residues, which are then conjugated with the preactivated APC to obtain the APC-antibody conjugate. |
T-EX7032: PE / R-Phycoerythrin Conjugation Kit |
T-EX7032: PE-Cy7 is a near-IR dye used primarily in flow cytometry for low-abundance antigens. Ex / Em: 569 nm / 780 nm |
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***) Gerne erstellen wir Ihnen ein detailliertes Angebot für Ihre Einkaufsabteilung. |
