Molecular Biology
Animal Tissue Direct PCR Kit
T-P-007 Animal Tissue Direct PCR Kit
Packaging: 100 T and 500 T are available
Product Description
The product is specially designed for amplification directly from animal tissues. Applicable samples include fresh or frozen mammalian viscera, nails, hair, zebrafish, drosophila, cultured cells, etc. The unique Animal Tissue Lysis Buffer A combine with Animal Tissue Lysis Buffer B to quickly release genomic DNA in micro tissues, without the need of genome extraction and purification, which is easy to operate, reduces the loss of samples, and greatly shortens the time-consuming experiments. The lysis product can be stored at -20 for a long time.
2× Animal Tissue Direct PCR Mix with Dye contains all components required for PCR except templates and primers. The DNA Polymerase is capable of amplifying rapidly and resist inhibitors after modification. The PCR Mix also contains a variety of reaction enhancers, which can further improve the amplification efficiency and fidelity, also amplify target genes up to 3 kb.
Storage and Shipping Conditions
Ship with wet ice; Animal Tissue Lysis Buffer A, stored at 4 ; Other components shall be stored at -20 ; Valid for 12 months.
Further similar Kits : Blood direct PCR Kit
Endo-Free Plasmid Miniprep Kit II
Description
The kit uses the traditional alkaline cleavage method to extract plasmid DNA of various transfection levels from bacteria. The silica gel membrane in the adsorption column of this kit is specially treated to greatly improve the adsorption effect of plasmid DNA, and 20-100 g plasmid DNA can be isolated from 5-15 ml Escherichia coli cultured overnight. In addition, Buffer ER and Buffer ED can effectively remove endotoxin (endotoxin content 0.1EU / g), protein and other impurities in plasmid DNA, and the extracted high-purity plasmid DNA can be directly used for transfection, enzyme digestion, transformation, PCR, sequencing and other molecular biology experiments..
Storage and Shipping Conditions
RNase A is shipped with wet ice and stored at -20°C. Other reagents are shipped and stored at room temperature; valid for 12 months.
Katalog Nummer: 25-5060 EXT; 50 T
DNA Bisulfite Conversion Kit
Conversion von nichtmethyliertem Cytosin in
Uracil : Tool zur Bestimmung epigenetischer Methylierung von DNA.
DNA Bisulfite Conversion Kit with Magnetic Beads provides a simple, rapid, efficient, high-throughput method for the conversion of unmethylated cytosines (C) of DNA into uracils by sodium bisulfite, while 5-methylcytosines (5mC) remain unchanged. The converted DNA can be purified with magnetic beads provided in the kit, and used for PCR amplification or high-throughput sequencing to detect and analyze DNA methylations.
This kit is ideal for high-throughput 96-well operations and is also suitable for routine single-tube operations. To facilitate 96-well operations, a 96-well deep well plate is supplied with this kit.
DNA methylation is an important epigenetic mechanism involving the transfer of a methyl group onto C5 position of the cytosine in DNA to form 5-methylcytosine (5mC) [1]. In eukaryotic genomes, the most common DNA modification is 5mC and CpG dinucleotides are the major methylation sites [2].
DNA methylation plays a significant role in regulating gene expression, maintaining chromatin structure, genomic imprinting, X chromosome inactivation, embryonic development, and other biological processes [3]. It has a profound impact on the onset and progression of aging and diseases [4]. It has been demonstrated that DNA methylation is closely related to various diseases, such as tumors, mental disorders, metabolic diseases, and autoimmune diseases [5]. DNA methylation can serve as a molecular marker or target to assist the precise diagnosis and treatment of diseases [4].
This product enables high cytosine conversion rates of over >99% with good reproducibility, making it the gold standard for DNA methylation analysis. When DNA is treated with sodium bisulfite, unmethylated cytosine undergoes sulphonation, deamination, desalting, and desulphonation, ultimately converting into uracil, while methylated cytosine remains in its original form.
Bisulfite conversion of cytosine (C) in DNA into uracil.
This method combines heat denaturation and bisulphite conversion steps for DNA treatment, followed by purification with magnetic beads. The desalination and desulphonation are completed during the DNA purification, making this method simpler and more convenient to use. It is suitable for automated high-throughput platforms and the whole process can be completed within 3 hours.
The workflow of DNA Bisulfite Conversion Kit with Magnetic Beads
The conversion products can be used for various downstream applications, such as methylation-specific PCR (MSP), bisulfite sequencing PCR (BSP), high-throughput sequencing, and restriction endonuclease analysis.
This kit enables high conversion rate and good DNA recovery. The applicable DNA amount is 10ng-2 g, with 0.5-2 g being more effective.
Katalog Nummer: 25-6010 DNA
DESCRIPTION
