Competent cells
Competent cells - E.coli strains
T-C1100 E.coli DH5α Competent Cells
€192,50
20x100µl
T-C1200 E.coli TOP10 Competent Cells
€192,50
20x100µl
T-C1300 E.coli JM109 Competent Cells
€192,50
20x100µl
T-C1400 E.coli BL21(DE3) Competent Cells
€192,50
20x100µl
T-C1500 BL21(DE3)pLysS Competent Cells
€192,50
20x100µl
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HT115(DE3) Competent Cells
HT115(DE3) Competent cells are usable for RNAi interference assays.
Cat: T-C3760
Size: 20×100μl
Price: 197,60 €
Storage: Store at -70°C, valid for 6 months.（Avoid repeated freezing and thawing）
Genotype: F-mcrA mcrB, IN(rrnD-rrnE)1 rnc14::Tn10λ(DE3 lavUV5::T7 polymerase)
Strain Resistance: Sensitive to ampicillin, kanamycin, chloramphenicol, and
streptomycin. Has tetracycline resistance.
Introduction:
HT115(DE3) is an RNase III defective Escherichia coli strain that can be expressed by IPTG
induction to produce dsRNA. It is used for RNAi interference assay. The strain also has the DE3
region of λ phage, which can express T7 RNA polymerase, and is suitable for the efficient
expression of prokaryotic expression vectors containing T7 promoters (such as pET, etc.). Non-T7
promoter expression vectors (such as pGEX, pMal, pTrc, etc.) can also be expressed in this strain.
HT115(DE3) competent cells were prepared by a special process. The conversion efficiency of
pUC19 plasmid was 1×106 cfu/μg DNA.
